Quantitative assessment of treatment response in Advanced Prostate Cancer (APC) with bone metastases remains an unmet clinical need. Whole-Body Diffusion-Weighted MRI (WB-DWI) provides two response biomarkers: Total Diffusion Volume (TDV) and global Apparent Diffusion Coefficient (gADC). However, tracking post-treatment changes of TDV and gADC from manually delineated lesions is cumbersome and increases inter-reader variability. We developed a software to automate this process. Core technologies include: (i) a weakly-supervised Residual U-Net model generating a skeleton probability map to isolate bone; (ii) a statistical framework for WB-DWI intensity normalisation, obtaining a signal-normalised b=900s/mm^2 (b900) image; and (iii) a shallow convolutional neural network that processes outputs from (i) and (ii) to generate a mask of suspected bone lesions, characterised by higher b900 signal intensity due to restricted water diffusion. This mask is applied to the gADC map to extract TDV and gADC statistics. We tested the tool using expert-defined metastatic bone disease delineations on 66 datasets, assessed repeatability of imaging biomarkers (N=10), and compared software-based response assessment with a construct reference standard (N=118). Average dice score between manual and automated delineations was 0.6 for lesions within pelvis and spine, with an average surface distance of 2mm. Relative differences for log-transformed TDV (log-TDV) and median gADC were 8.8% and 5%, respectively. Repeatability analysis showed coefficients of variation of 4.6% for log-TDV and 3.5% for median gADC, with intraclass correlation coefficients of 0.94 or higher. The software achieved 80.5% accuracy, 84.3% sensitivity, and 85.7% specificity in assessing response to treatment. Average computation time was 90s per scan.

Accurate and scalable cancer diagnosis remains a critical challenge in modern pathology, particularly for malignancies such as breast, prostate, bone, and cervical, which exhibit complex histological variability. In this study, we propose a transformer-based deep learning framework for multi-class tumor classification in histopathological images. Leveraging a fine-tuned Vision Transformer (ViT) architecture, our method addresses key limitations of conventional convolutional neural networks, offering improved performance, reduced preprocessing requirements, and enhanced scalability across tissue types. To adapt the model for histopathological cancer images, we implement a streamlined preprocessing pipeline that converts tiled whole-slide images into PyTorch tensors and standardizes them through data normalization. This ensures compatibility with the ViT architecture and enhances both convergence stability and overall classification performance. We evaluate our model on four benchmark datasets: ICIAR2018 (breast), SICAPv2 (prostate), UT-Osteosarcoma (bone), and SipakMed (cervical) dataset -- demonstrating consistent outperformance over existing deep learning methods. Our approach achieves classification accuracies of 99.32%, 96.92%, 95.28%, and 96.94% for breast, prostate, bone, and cervical cancers respectively, with area under the ROC curve (AUC) scores exceeding 99% across all datasets. These results confirm the robustness, generalizability, and clinical potential of transformer-based architectures in digital pathology. Our work represents a significant advancement toward reliable, automated, and interpretable cancer diagnosis systems that can alleviate diagnostic burdens and improve healthcare outcomes.

Ewing's sarcoma (ES), characterized by a high density of small round blue cells without structural organization, presents a significant health concern, particularly among adolescents aged 10 to 19. Artificial intelligence-based systems for automated analysis of histopathological images are promising to contribute to an accurate diagnosis of ES. In this context, this study explores the feature extraction ability of different pre-training strategies for distinguishing ES from other soft tissue or bone sarcomas with similar morphology in digitized tissue microarrays for the first time, as far as we know. Vision-language supervision (VLS) is compared to fully-supervised ImageNet pre-training within a multiple instance learning paradigm. Our findings indicate a substantial improvement in diagnostic accuracy with the adaption of VLS using an in-domain dataset. Notably, these models not only enhance the accuracy of predicted classes but also drastically reduce the number of trainable parameters and computational costs.

Recent advances in machine learning are transforming medical image analysis, particularly in cancer detection and classification. Techniques such as deep learning, especially convolutional neural networks (CNNs) and vision transformers (ViTs), are now enabling the precise analysis of complex histopathological images, automating detection, and enhancing classification accuracy across various cancer types. This study focuses on osteosarcoma (OS), the most common bone cancer in children and adolescents, which affects the long bones of the arms and legs. Early and accurate detection of OS is essential for improving patient outcomes and reducing mortality. However, the increasing prevalence of cancer and the demand for personalized treatments create challenges in achieving precise diagnoses and customized therapies. We propose a novel hybrid model that combines convolutional neural networks (CNN) and vision transformers (ViT) to improve diagnostic accuracy for OS using hematoxylin and eosin (H&E) stained histopathological images. The CNN model extracts local features, while the ViT captures global patterns from histopathological images. These features are combined and classified using a Multi-Layer Perceptron (MLP) into four categories: non-tumor (NT), non-viable tumor (NVT), viable tumor (VT), and none-viable ratio (NVR). Using the Cancer Imaging Archive (TCIA) dataset, the model achieved an accuracy of 99.08%, precision of 99.10%, recall of 99.28%, and an F1-score of 99.23%. This is the first successful four-class classification using this dataset, setting a new benchmark in OS research and offering promising potential for future diagnostic advancements.

Purpose: Bone metastasis have a major impact on the quality of life of patients and they are diverse in terms of size and location, making their segmentation complex. Manual segmentation is time-consuming, and expert segmentations are subject to operator variability, which makes obtaining accurate and reproducible segmentations of bone metastasis on CT-scans a challenging yet important task to achieve. Materials and Methods: Deep learning methods tackle segmentation tasks efficiently but require large datasets along with expert manual segmentations to generalize on new images. We propose an automated data synthesis pipeline using 3D Denoising Diffusion Probabilistic Models (DDPM) to enchance the segmentation of femoral metastasis from CT-scan volumes of patients. We used 29 existing lesions along with 26 healthy femurs to create new realistic synthetic metastatic images, and trained a DDPM to improve the diversity and realism of the simulated volumes. We also investigated the operator variability on manual segmentation. Results: We created 5675 new volumes, then trained 3D U-Net segmentation models on real and synthetic data to compare segmentation performance, and we evaluated the performance of the models depending on the amount of synthetic data used in training. Conclusion: Our results showed that segmentation models trained with synthetic data outperformed those trained on real volumes only, and that those models perform especially well when considering operator variability.

Back in sixth grade, I was a robot. Or at least, that's what I told anyone who asked--in reality, my 11-year-old self was completely human. In 2018, I was diagnosed with osteosarcoma, a rare bone cancer that meant nine months of chemotherapy and too many surgeries to count. It was a year punctuated with hospital visits, needle pokes, and days when I felt too nauseated to even look at a plate of food. And yet, my primary concern was that in my immunocompromised state, I was no longer able to attend school.